IL2 Receptor beta ELISA Kit
Quick Overview for IL2 Receptor beta ELISA Kit (ABIN7830604)
Target
See all IL2 Receptor beta (IL2RB) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- IL2RB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL2RB expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Interleukin 2 Receptor, beta (IL2RB) ELISA Kit
IL2RB Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, beta (IL2RB) ELISA KitIL2RB Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 2 Receptor, beta (IL2RB) ELISA KitIL2RB Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))
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Alternative Name
- IL2RB
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Background
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Synonyms: CD122|P70-75
Gene Description: interleukin 2 receptor, beta
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Gene ID
- 3560
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UniProt
- P14784
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Pathways
- JAK-STAT Signaling, Growth Factor Binding
Target See all IL2 Receptor beta (IL2RB) ELISA Kits
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