EPH Receptor A5 ELISA Kit

Catalog No. ABIN7830625

Human EPH Receptor A5 ELISA Kit, Colorimetric assay for quantification of Human EPH Receptor A5.

Quick Overview for EPH Receptor A5 ELISA Kit (ABIN7830625)

Target: EPH Receptor A5 (EPHA5)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details EPH Receptor A5 ELISA Kit

Purpose: EPHA5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPHA5 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for EPH Receptor A5

Target: EPH Receptor A5 (EPHA5)

Alternative Name: EPHA5

Background:

Synonyms: CEK7|EHK1|HEK7|TYRO4

Gene Description: EPH receptor A5

Gene ID: 2044

UniProt: P54756

Pathways: RTK Signaling