MAPK6 ELISA Kit

Catalog No. ABIN7830634

Human MAPK6 ELISA Kit, Colorimetric assay for quantification of Human MAPK6.

Quick Overview for MAPK6 ELISA Kit (ABIN7830634)

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MAPK6 ELISA Kit

Purpose: MAPK6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAPK6 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Alternative Name: MAPK6

Background:

Synonyms: DKFZp686F03189|ERK3|HsT17250|PRKM6|p97MAPK

Gene Description: mitogen-activated protein kinase 6

Gene ID: 5597

UniProt: Q16659

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C