Estrogen Receptor alpha ELISA Kit

Catalog No. ABIN7830636

The Human Estrogen Receptor alpha ELISA Kit (ABIN7830636) is a Colorimetric ELISA Kit designed to quantify Human Estrogen Receptor alpha.

Quick Overview for Estrogen Receptor alpha ELISA Kit (ABIN7830636)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Estrogen Receptor alpha ELISA Kit

Purpose: ESR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ESR1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background:

Synonyms: DKFZp686N23123|ER|ESR|ESRA|Era|NR3A1

Gene Description: estrogen receptor 1

Gene ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly