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FLT3 ELISA Kit

Human FLT3 ELISA Kit, Colorimetric assay for quantification of Human FLT3.
Catalog No. ABIN7830655
$870.57
Plus shipping costs $50.00
1 kit
Shipping to: United States
Delivery in 8 to 11 Business Days

Quick Overview for FLT3 ELISA Kit (ABIN7830655)

Target

See all FLT3 ELISA Kits
FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity

  • 12
  • 4
  • 1
Human

Detection Method

Colorimetric

Method Type

Cell ELISA

Application

ELISA

Sample Type

Cell Samples, Suspension Cell Culture
  • Purpose

    FLT3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FLT3 expression in cultured cells.

    Analytical Method

    Qualitative

    Cross-Reactivity

    Mouse
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Plate

    Uncoated

    Protocol

    1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
    2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
    3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
    4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

    Restrictions

    For Research Use only
  • Storage

    4 °C

    Storage Comment

    Store the kit at 4°C.
  • Target See all FLT3 ELISA Kits

    FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

    Alternative Name

    FLT3

    Background

    Synonyms: CD135|FLK2|STK1

    Gene Description: fms-related tyrosine kinase 3

    Gene ID

    2322

    UniProt

    P36888

    Pathways

    RTK Signaling
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