Galectin 3 ELISA Kit
Quick Overview for Galectin 3 ELISA Kit (ABIN7830669)
Target
See all Galectin 3 (LGALS3) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- LGALS3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LGALS3 expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- Galectin 3 (LGALS3)
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Alternative Name
- LGALS3
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Background
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Synonyms: CBP35|GAL3|GALBP|GALIG|LGALS2|MAC2
Gene Description: lectin, galactoside-binding, soluble, 3
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Gene ID
- 3958
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UniProt
- P17931
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Pathways
- RTK Signaling
Target See all Galectin 3 (LGALS3) ELISA Kits
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