GSK3 alpha ELISA Kit
Quick Overview for GSK3 alpha ELISA Kit (ABIN7830689)
Target
See all GSK3 alpha (GSK3a) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- GSK3A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GSK3A expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse, Rat
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Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA Kit
GSK3a Reactivity: Mouse Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a Reactivity: Rat Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
Glycogen Synthase Kinase 3 alpha (GSK3a) ELISA KitGSK3a Reactivity: Rat Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
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Alternative Name
- GSK3A
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Background
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Synonyms: DKFZp686D0638
Gene Description: glycogen synthase kinase 3 alpha
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Gene ID
- 2931
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UniProt
- P49840
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Pathways
- PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
Target See all GSK3 alpha (GSK3a) ELISA Kits
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