MEF2A ELISA Kit

Catalog No. ABIN7830756

The Human MEF2A ELISA Kit (ABIN7830756) is a Colorimetric ELISA Kit designed to quantify Human MEF2A.

Quick Overview for MEF2A ELISA Kit (ABIN7830756)

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MEF2A ELISA Kit

Purpose: MEF2A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MEF2A expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for MEF2A

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Alternative Name: MEF2A

Background:

Synonyms: ADCAD1|RSRFC4|RSRFC9

Gene Description: myocyte enhancer factor 2A

Gene ID: 4205

UniProt: Q02078

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades