CDKN1C ELISA Kit

Catalog No. ABIN7830790

Human CDKN1C ELISA Kit, Colorimetric assay for quantification of Human CDKN1C.

Quick Overview for CDKN1C ELISA Kit (ABIN7830790)

Target: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CDKN1C ELISA Kit

Purpose: CDKN1C (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CDKN1C expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for CDKN1C

Target: CDKN1C (Cyclin-Dependent Kinase Inhibitor 1C (p57, Kip2) (CDKN1C))

Alternative Name: CDKN1C

Background:

Synonyms: BWCR|BWS|KIP2|WBS|p57

Gene Description: cyclin-dependent kinase inhibitor 1C (p57, Kip2)

Gene ID: 1028

Pathways: Cell Division Cycle, Dopaminergic Neurogenesis