GRIN1/NMDAR1 ELISA Kit

Catalog No. ABIN7830801

The Human GRIN1/NMDAR1 ELISA Kit (ABIN7830801) is a Colorimetric ELISA Kit designed to quantify Human GRIN1/NMDAR1.

Quick Overview for GRIN1/NMDAR1 ELISA Kit (ABIN7830801)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details GRIN1/NMDAR1 ELISA Kit

Purpose: GRIN1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GRIN1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1

Background:

Synonyms: NMDA1|NMDAR1|NR1

Gene Description: glutamate receptor, ionotropic, N-methyl D-aspartate 1

Gene ID: 2902

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity