GRIN2B ELISA Kit

Catalog No. ABIN7830802

Human GRIN2B ELISA Kit, Colorimetric assay for quantification of Human GRIN2B.

Quick Overview for GRIN2B ELISA Kit (ABIN7830802)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details GRIN2B ELISA Kit

Purpose: GRIN2B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GRIN2B expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: GRIN2B

Background:

Synonyms: MGC142178|MGC142180|NMDAR2B|NR2B|hNR3

Gene Description: glutamate receptor, ionotropic, N-methyl D-aspartate 2B

Gene ID: 2904

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity