PML ELISA Kit

Catalog No. ABIN7830846

The Human PML ELISA Kit (ABIN7830846) is a Colorimetric ELISA Kit designed to quantify Human PML.

Quick Overview for PML ELISA Kit (ABIN7830846)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details PML ELISA Kit

Purpose: PML (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PML expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: PML

Background:

Synonyms: MYL|PP8675|RNF71|TRIM19

Gene Description: promyelocytic leukemia

Gene ID: 5371

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus