Patched 1 ELISA Kit

Catalog No. ABIN7830864

The Human Patched 1 ELISA Kit (ABIN7830864) is a Colorimetric ELISA Kit designed to quantify Human Patched 1.

Quick Overview for Patched 1 ELISA Kit (ABIN7830864)

Target: Patched 1 (PTCH1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Patched 1 ELISA Kit

Purpose: PTCH1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PTCH1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1

Background:

Synonyms: BCNS|FLJ26746|FLJ42602|HPE7|NBCCS|PTC|PTC1|PTCH|PTCH11

Gene Description: patched homolog 1 (Drosophila)

Gene ID: 5727

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation