TEK ELISA Kit
Quick Overview for TEK ELISA Kit (ABIN7830910)
Target
See all TEK ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- TEK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TEK expression in cultured cells.
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Analytical Method
- Qualitative
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Cross-Reactivity
- Mouse
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store the kit at 4°C.
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- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
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Alternative Name
- TEK
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Background
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Synonyms: CD202B|TIE-2|TIE2|VMCM|VMCM1
Gene Description: TEK tyrosine kinase, endothelial
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Gene ID
- 7010
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Pathways
- RTK Signaling, Growth Factor Binding
Target See all TEK ELISA Kits
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