Topoisomerase II alpha ELISA Kit

Catalog No. ABIN7830920

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Topoisomerase II alpha.

Quick Overview for Topoisomerase II alpha ELISA Kit (ABIN7830920)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Topoisomerase II alpha ELISA Kit

Purpose: TOP2A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TOP2A expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: TOP2A

Background:

Synonyms: TOP2|TP2A

Gene Description: topoisomerase (DNA) II alpha 170 kDa

Gene ID: 7153

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases