KAT2B ELISA Kit

Catalog No. ABIN7830952

Human KAT2B ELISA Kit, Colorimetric assay for quantification of Human KAT2B.

Quick Overview for KAT2B ELISA Kit (ABIN7830952)

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details KAT2B ELISA Kit

Purpose: KAT2B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of KAT2B expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Alternative Name: KAT2B

Background:

Synonyms: CAF|P|P/CAF|PCAF

Gene Description: K(lysine) acetyltransferase 2B

Gene ID: 8850

Pathways: p53 Signaling, Regulation of Carbohydrate Metabolic Process