MSK1 ELISA Kit

Catalog No. ABIN7830957

The Human MSK1 ELISA Kit (ABIN7830957) is a Colorimetric ELISA Kit designed to quantify Human MSK1.

Quick Overview for MSK1 ELISA Kit (ABIN7830957)

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MSK1 ELISA Kit

Purpose: RPS6KA5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPS6KA5 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for MSK1

Target: MSK1 (RPS6KA5) (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5))

Alternative Name: RPS6KA5

Background:

Synonyms: MGC1911|MSK1|MSPK1|RLPK

Gene Description: ribosomal protein S6 kinase, 90 kDa, polypeptide 5

Gene ID: 9252

Pathways: MAPK Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades