RPS6KA1 ELISA Kit

Catalog No. ABIN7830975

The Human RPS6KA1 ELISA Kit (ABIN7830975) is a Colorimetric ELISA Kit designed to quantify Human RPS6KA1.

Quick Overview for RPS6KA1 ELISA Kit (ABIN7830975)

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details RPS6KA1 ELISA Kit

Purpose: RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of RPS6KA1/RPS6KA3/RPS6KA2/RPS6KA6 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for RPS6KA1

Target: RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

Alternative Name: RPS6KA1

Background:

Synonyms: HU-1|MAPKAPK1A|RSK|RSK1

Gene Description: ribosomal protein S6 kinase, 90 kDa, polypeptide 1

Gene ID: 6195

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades