MKNK1 ELISA Kit

Catalog No. ABIN7830980

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MKNK1.

Quick Overview for MKNK1 ELISA Kit (ABIN7830980)

Target: MKNK1 (MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MKNK1 ELISA Kit

Purpose: MKNK1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MNK1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for MKNK1

Target: MKNK1 (MAP Kinase Interacting serine/threonine Kinase 1 (MKNK1))

Alternative Name: MKNK1

Background:

Synonyms: MNK1

Gene Description: MAP kinase interacting serine/threonine kinase 1

Gene ID: 8569

UniProt: Q9BUB5

Pathways: MAPK Signaling, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor