Mre11 ELISA Kit

Catalog No. ABIN7830982

Human Mre11 ELISA Kit, Colorimetric assay for quantification of Human Mre11.

Quick Overview for Mre11 ELISA Kit (ABIN7830982)

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details Mre11 ELISA Kit

Purpose: MRE11A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MRE11 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Alternative Name: MRE11A

Background:

Synonyms: ATLD|HNGS1|MRE11|MRE11B

Gene Description: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)

Gene ID: 4361

UniProt: P49959

Pathways: DNA Damage Repair