NEK9 ELISA Kit

Catalog No. ABIN7830984

The Human NEK9 ELISA Kit (ABIN7830984) is a Colorimetric ELISA Kit designed to quantify Human NEK9.

Quick Overview for NEK9 ELISA Kit (ABIN7830984)

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details NEK9 ELISA Kit

Purpose: NEK9 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NEK9 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for NEK9

Target: NEK9 (NIMA (Never in Mitosis Gene A)- Related Kinase 9 (NEK9))

Alternative Name: NEK9

Background:

Synonyms: DKFZp434D0935|MGC138306|MGC16714|NERCC|NERCC1|Nek8

Gene Description: NIMA (never in mitosis gene a)- related kinase 9

Gene ID: 91754

UniProt: Q8TD19

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection