NCF1 ELISA Kit

Catalog No. ABIN7830989

This Colorimetric ELISA kit is designed for the quantitative measurement of Human NCF1.

Quick Overview for NCF1 ELISA Kit (ABIN7830989)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details NCF1 ELISA Kit

Purpose: NCF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of p47 phox expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: NCF1

Background:

Synonyms: FLJ79451|NCF1A|NOXO2|SH3PXD1A|p47phox

Gene Description: neutrophil cytosolic factor 1

Gene ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signaling