MID1 ELISA Kit

Catalog No. ABIN7831033

Human MID1 ELISA Kit, Colorimetric assay for quantification of Human MID1.

Quick Overview for MID1 ELISA Kit (ABIN7831033)

Target: MID1 (Midline 1 (MID1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MID1 ELISA Kit

Purpose: MID1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MID1 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for MID1

Target: MID1 (Midline 1 (MID1))

Alternative Name: MID1

Background:

Synonyms: BBBG1|FXY|GBBB1|MIDIN|OGS1|OS|OSX|RNF59|TRIM18|XPRF|ZNFXY

Gene Description: midline 1 (Opitz/BBB syndrome)

Gene ID: 4281

UniProt: O15344