T-Box 1 ELISA Kit

Catalog No. ABIN7831037

This Colorimetric ELISA kit is designed for the quantitative measurement of Human T-Box 1.

Quick Overview for T-Box 1 ELISA Kit (ABIN7831037)

Target: T-Box 1 (TBX1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details T-Box 1 ELISA Kit

Purpose: TBX1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TBX1 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for T-Box 1

Target: T-Box 1 (TBX1)

Alternative Name: TBX1

Background:

Synonyms: CAFS|CTHM|DGCR|DGS|DORV|TBX1C|TGA|VCFS

Gene Description: T-box 1

Gene ID: 6899

UniProt: O43435

Pathways: Retinoic Acid Receptor Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation