ICAM1 ELISA Kit
Quick Overview for ICAM1 ELISA Kit (ABIN7832841)
Target
See all ICAM1 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA ICAM-1/CD54 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human ICAM-1/CD54 (intercellular adhesion molecule 1) in serum, plasma and other biological fluids.
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Analytical Method
- Quantitative
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Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit
ICAM1 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Rabbit Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Dog Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Human AA 28-480 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Mouse AA 28-485 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Rat AA 28-493 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Intercellular Adhesion Molecule 1 (ICAM1) ELISA KitICAM1 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C
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Storage Comment
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
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Alternative Name
- ICAM-1/CD54
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
Target See all ICAM1 ELISA Kits
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