ADIPOQ ELISA Kit
Quick Overview for ADIPOQ ELISA Kit (ABIN7832851)
Target
See all ADIPOQ ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA ADIPOQ (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human ADIPOQ (Adiponectin) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human ADIPOQ. No significant cross-reactivity or interference between Human ADIPOQ and analogues was observed.
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Adiponectin (ADIPOQ) ELISA Kit
ADIPOQ Reactivity: Human Colorimetric Sandwich ELISA 1.562-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Sheep Colorimetric Competition ELISA 6.25 ng/mL - 100 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Rabbit Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Chicken Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Pig Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adiponectin (ADIPOQ) ELISA KitADIPOQ Reactivity: Human AA 19-244 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- ADIPOQ (Adiponectin (ADIPOQ))
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Alternative Name
- ADIPOQ
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Background
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Synonyms: ACDC|ACRP30|ADPN|APM-1|APM1|GBP28|adiponectin
Gene Description: adiponectin, C1Q and collagen domain containing
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Gene ID
- 9370
Target See all ADIPOQ ELISA Kits
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