CRP ELISA Kit
Quick Overview for CRP ELISA Kit (ABIN7832856)
Target
See all CRP ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
-
-
Purpose
- FastELISA CRP (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human CRP (C-reactive protein) in Serum, plasma.
-
Analytical Method
- Quantitative
-
Specificity
- This assay is highly specific for detection of Human CRP. No significant cross-reactivity or interference between Human CRP and analogues was observed.
-
-
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Pre-coated
-
Protocol
-
- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- Avoid repeated freezing and thawing
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
-
Expiry Date
- 6 months
-
-
- CRP (C-Reactive Protein (CRP))
-
Alternative Name
- CRP
-
Background
-
Synonyms: MGC149895|MGC88244|PTX1
Gene Description: C-reactive protein, pentraxin-related
-
Gene ID
- 1401
-
Pathways
- Carbohydrate Homeostasis
Target See all CRP ELISA Kits
-