Ephrin A1 ELISA Kit
Quick Overview for Ephrin A1 ELISA Kit (ABIN7832859)
Target
See all Ephrin A1 (EFNA1) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA EFNA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human EFNA1 (Ephrin A1) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human EFNA1. No significant cross-reactivity or interference between Human EFNA1 and analogues was observed.
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Ephrin A1 (EFNA1) ELISA Kit
EFNA1 Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Ephrin A1 (EFNA1) ELISA KitEFNA1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ephrin A1 (EFNA1) ELISA KitEFNA1 Reactivity: Mouse Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Ephrin A1 (EFNA1) ELISA KitEFNA1 Reactivity: Human Colorimetric Sandwich ELISA 78.13-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Ephrin A1 (EFNA1) ELISA KitEFNA1 Reactivity: Mouse Colorimetric Sandwich ELISA 93.75-6000 pg/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- Ephrin A1 (EFNA1)
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Alternative Name
- EFNA1
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Background
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Synonyms: B61|ECKLG|EFL1|EPLG1|LERK1|TNFAIP4
Gene Description: ephrin-A1
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Gene ID
- 1942
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Pathways
- RTK Signaling
Target See all Ephrin A1 (EFNA1) ELISA Kits
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