GDF15 ELISA Kit
Quick Overview for GDF15 ELISA Kit (ABIN7832861)
Target
See all GDF15 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA GDF15 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human GDF15 (Growth differentiation factor 15) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human GDF15. No significant cross-reactivity or interference between Human GDF15 and analogues was observed.
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Growth Differentiation Factor 15 (GDF15) ELISA Kit
GDF15 Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate, Urine
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Human Colorimetric Sandwich ELISA 23.438 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Mouse Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Rat Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Human Colorimetric Sandwich ELISA 8-500 pg/mL Cell Lysate, Plasma, Serum
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Human Colorimetric Sandwich ELISA 23.438 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Growth Differentiation Factor 15 (GDF15) ELISA KitGDF15 Reactivity: Human AA 197-308 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- GDF15 (Growth Differentiation Factor 15 (GDF15))
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Alternative Name
- GDF15
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Background
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Synonyms: GDF-15|MIC-1|MIC1|NAG-1|PDF|PLAB|PTGFB
Gene Description: growth differentiation factor 15
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Gene ID
- 9518
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Pathways
- SARS-CoV-2 Protein Interactome
Target See all GDF15 ELISA Kits
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