NLRP3 ELISA Kit
Quick Overview for NLRP3 ELISA Kit (ABIN7832872)
Target
See all NLRP3 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA NLRP3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human NLRP3 (NLR family pyrin domain containing 3) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human NLRP3. No significant cross-reactivity or interference between Human NLRP3 and analogues was observed.
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NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA Kit
NLRP3 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
NLR Family, Pyrin Domain Containing 3 (NLRP3) ELISA KitNLRP3 Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))
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Alternative Name
- NLRP3
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Background
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Synonyms: AGTAVPRL|AII|AII/AVP|AVP|C1orf7|CIAS1|CLR1.1|FCAS|FCU|FLJ95925|MWS|NALP3|PYPAF1
Gene Description: NLR family, pyrin domain containing 3
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Gene ID
- 114548
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome
Target See all NLRP3 ELISA Kits
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