SAA1 ELISA Kit
Quick Overview for SAA1 ELISA Kit (ABIN7832881)
Target
See all SAA1 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA SAA (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human SAA (Serum Amyloid A) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human SAA. No significant cross-reactivity or interference between Human SAA and analogues was observed.
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Serum Amyloid A1 (SAA1) ELISA Kit
SAA1 Reactivity: Human Colorimetric Sandwich ELISA 156 ng/mL - 10000 ng/mL Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Monkey Colorimetric Competition ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Sheep Colorimetric Competition ELISA 1.875 ng/mL - 120 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Mouse AA 20-122 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Human AA 20-122 Colorimetric Sandwich ELISA
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Monkey Colorimetric Sandwich ELISA 0.625-40 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- SAA1 (Serum Amyloid A1 (SAA1))
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Alternative Name
- SAA1
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Background
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Synonyms: MGC111216|PIG4|SAA|TP53I4
Gene Description: serum amyloid A1
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Gene ID
- 6288
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Pathways
- Toll-Like Receptors Cascades
Target See all SAA1 ELISA Kits
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