SAA1 ELISA Kit
Quick Overview for SAA1 ELISA Kit (ABIN7832976)
Target
See all SAA1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 312 pg/mL
-
Purpose
- Sandwich ELISA for quantitative detection of Human SAA1 concentrations.
-
Analytical Method
- Quantitative
-
Sensitivity
- 125 pg/mL
-
Components
-
- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
-
Material not included
-
- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
-
-
Serum Amyloid A1 (SAA1) ELISA Kit
SAA1 Reactivity: Human Colorimetric Sandwich ELISA 156 ng/mL - 10000 ng/mL Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Monkey Colorimetric Competition ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Sheep Colorimetric Competition ELISA 1.875 ng/mL - 120 ng/mL Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.312-20 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Mouse AA 20-122 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Human AA 20-122 Colorimetric Sandwich ELISA
Serum Amyloid A1 (SAA1) ELISA KitSAA1 Reactivity: Monkey Colorimetric Sandwich ELISA 0.625-40 ng/mL Plasma, Serum, Tissue Homogenate
-
-
Sample Volume
- 100 μL
-
Plate
- Pre-coated
-
Protocol
-
- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- Avoid freeze/thaw cycles
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
-
Expiry Date
- 12 months
-
-
- SAA1 (Serum Amyloid A1 (SAA1))
-
Alternative Name
- SAA1
-
Background
- Alternative Names: Serum amyloid A-1 protein, SAA
-
Gene ID
- 6288
-
UniProt
- P0DJI8
-
Pathways
- Toll-Like Receptors Cascades
Target See all SAA1 ELISA Kits
-
