P4HB ELISA Kit

Catalog No. ABIN7833054

Human P4HB ELISA Kit, Colorimetric assay for quantification of Human P4HB.

Quick Overview for P4HB ELISA Kit (ABIN7833054)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Plasma, Serum

Product Details P4HB ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich ELISA for quantitative detection of Human P4HB concentrations.

Analytical Method: Quantitative

Sensitivity: 127 pg/mL

Components:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Material not included:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Restrictions: For Research Use only

Handling

Handling Advice: Avoid freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: P4HB

Background: Alternative Names: ERBA2L, PDI, PDIA1, PO4DB, Protein disulfide-isomerase, PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism