CD130/gp130 ELISA Kit
Quick Overview for CD130/gp130 ELISA Kit (7833099)
Target
See all CD130/gp130 (IL6ST) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
-
-
Minimum Detection Limit
- 3.12 ng/mL
-
Purpose
- Sandwich ELISA for quantitative detection of Rat CD130 concentrations.
-
Analytical Method
- Quantitative
-
Sensitivity
- 1210 pg/mL
-
Components
-
- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
-
Material not included
-
- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
-
-
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA Kit
IL6ST Reactivity: Human Colorimetric Sandwich ELISA 0.938-60 ng/mL Plasma, Saliva, Serum, Tissue Homogenate, Urine
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Rat Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Mouse Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Rat Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Mouse Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum
Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST) ELISA KitIL6ST Reactivity: Human AA 24-619 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
-
-
Sample Volume
- 100 μL
-
Plate
- Pre-coated
-
Protocol
-
- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- Avoid freeze/thaw cycles
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
-
Expiry Date
- 12 months
-
-
- CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))
-
Alternative Name
- IL6ST
-
Background
- Alternative Names: Interleukin-6 receptor subunit beta, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6R-beta, IL-6RB, CDw130, Interleukin-6 signal transducer, Membrane glycoprotein 130, gp130, Oncostatin-M receptor subunit alpha, CD130
-
Gene ID
- 25205
-
UniProt
- P40190
-
Pathways
- JAK-STAT Signaling, Cellular Glucan Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Cancer Immune Checkpoints
Target See all CD130/gp130 (IL6ST) ELISA Kits
-
