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IGFBP4 ELISA Kit

Human IGFBP4 ELISA Kit, Colorimetric assay for quantification of Human IGFBP4.
Catalog No. ABIN7849866
$660.00
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 12 to 15 Business Days

Quick Overview for IGFBP4 ELISA Kit (ABIN7849866)

Target

See all IGFBP4 ELISA Kits
IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Reactivity

  • 11
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

390 pg/mL - 25000 pg/mL

Application

ELISA

Sample Type

Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
  • Minimum Detection Limit

    390 pg/mL

    Purpose

    For quantitative detection of IGFBP4 in human serum, plasma, tissuehomogenateor cell culture supernatants.

    Analytical Method

    Quantitative

    Sensitivity

    < 50 pg/mL

    Material not included

    1. 37 °C incubator
    2. Microplate reader (wavelength: 450 nm)
    3. Precise pipette and disposable pipette tips
    4. Automated plate washer
    5. ELISA shaker
    6. 1.5 mL of Eppendorf tubes
    7. Plate cover
    8. Absorbent filter papers
    9. Plastic or glass container with volume of above 1 L
  • Plate

    Pre-coated

    Reagent Preparation

    1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

    Restrictions

    For Research Use only
  • Storage

    4 °C,-20 °C

    Storage Comment

    Store at 2-8°C for 6 months, or at -20°C for 12 months.

    Expiry Date

    12 months
  • Target See all IGFBP4 ELISA Kits

    IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

    Alternative Name

    IGFBP4

    Pathways

    WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process
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