Adenosine Diphosphate (ADP) Fluorometric Assay Kit

Catalog No. ABIN7849934

Quick Overview for Adenosine Diphosphate (ADP) Fluorometric Assay Kit (ABIN7849934)

Target: ADP (Adenosine Diphosphate (ADP))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 2.8 μmol/L - 50 μmol/L

Minimum Detection Limit: 2.8 μmol/L

Purpose: Adenosine Diphosphate (ADP) Fluorometric Assay Kit

Characteristics: Adenosine diphosphate (ADP) is a nucleoside that plays a key role in energy transfer reactions. In organisms, ADP is usually the product of Adenosine triphosphate (ATP) which hydrolysis after the loss of a phosphate group, that is breaking a high-energy phosphate bond and releasing energy. ADP exists in high-density particles in platelet cells and is released when platelets undergo coagulation reaction. ADP affects the shape and biological behavior of platelets through ADP receptors on platelets, and further accelerates the process of platelet coagulation. The detection principle of this kit is that ADP and the substrate are catalyzed by enzyme reagents to make the chromogenic agent produce fluorescence. The fluorescence microplate reader was used to detect the excitation wavelength of 535 nm and the emission wavelength of 587nm, and the ADP content in the sample was calculated according to the standard curve.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 4.4-9.6%

Intra-assay precision: 4.0-5.0%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: ADP (Adenosine Diphosphate (ADP))

Alternative Name: ADP