Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Catalog No. ABIN7849938

Quick Overview for Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit (ABIN7849938)

Target: ALT (Alanine Aminotransferase (ALT))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.01 U/L - 0.83 U/L

Minimum Detection Limit: 0.01 U/L

Purpose: Alanine Aminotransferase (ALT/GPT) Activity Fluorometric Assay Kit

Characteristics: ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 590 nm.

Application Details

Assay Time: 1.5 h

Assay Precision:

Inter-assay precision: 9.8%

Intra-assay precision: 2.3%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: ALT (Alanine Aminotransferase (ALT))

Alternative Name: ALT