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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

BCA Colorimetric Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Samples Quantitative
Catalog No. ABIN7849939
$636.00
Plus shipping costs $50.00
500 tests
Shipping to: United States
Delivery in 12 to 15 Business Days

Quick Overview for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (ABIN7849939)

Target

See all ALT Kits
ALT (Alanine Aminotransferase (ALT))

Detection Method

Colorimetric

Application

Biochemical Assay (BCA)

Sample Type

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method

Quantitative
  • Detection Range

    0.75 IU/L - 72.3 IU/L

    Minimum Detection Limit

    0.75 IU/L

    Purpose

    Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

    Characteristics

    ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37 °C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
  • Assay Time

    1.5 h

    Assay Precision

    Inter-assay precision: 9%

    Intra-assay precision: 5.3%

    Restrictions

    For Research Use only
  • Handling Advice

    Protect from light.

    Storage

    4 °C

    Storage Comment

    This product can be stored at 2~8°C for 12 months with shading light.

    Expiry Date

    12 months
  • Target

    ALT (Alanine Aminotransferase (ALT))

    Alternative Name

    ALT
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