Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
Quick Overview for Alanine Aminotransferase (ALT/GPT) Activity Assay Kit (ABIN7849939)
Target
See all ALT KitsDetection Method
Application
Sample Type
Analytical Method
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Detection Range
- 0.75 IU/L - 72.3 IU/L
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Minimum Detection Limit
- 0.75 IU/L
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Purpose
- Alanine Aminotransferase (ALT/GPT) Activity Assay Kit
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Characteristics
- ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37 °C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
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Assay Time
- 1.5 h
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Assay Precision
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Inter-assay precision: 9%
Intra-assay precision: 5.3%
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- 4 °C
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Storage Comment
- This product can be stored at 2~8°C for 12 months with shading light.
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Expiry Date
- 12 months
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- ALT (Alanine Aminotransferase (ALT))
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Alternative Name
- ALT
Target
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