Phosphofructokinase(PFK)Activity Assay Kit

Catalog No. ABIN7849955

Quick Overview for Phosphofructokinase(PFK)Activity Assay Kit (ABIN7849955)

Target: ATP-Dependent 6-Phosphofructokinase

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.27 U/L - 32.29 U/L

Minimum Detection Limit: 0.27 U/L

Purpose: Phosphofructokinase(PFK)Activity Assay Kit

Characteristics: Phosphofructokinase(also known as 6-phosphofructokinase, PFK) is a class of kinases that act on fructose-6-phosphate. Phosphofructokinase catalyzes fructose-6-phosphate and ATP to produce fructose-1, 6-diphosphate and ADP, and pyruvate kinase and lactate dehydrogenase further catalyze the oxidation of NADH to NAD+. The activity of PFK can be reflected by the determination of NADH decline rate at 340 nm.

Application Details

Assay Time: 15 min

Assay Precision:

Inter-assay precision: 8%

Intra-assay precision: 3%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: ATP-Dependent 6-Phosphofructokinase

Alternative Name: PFK