β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit

Catalog No. ABIN7849957

Quick Overview for β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit (ABIN7849957)

Target: BACE1 (Beta-secretase 1 (BACE1))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.003 U/L - 4.75 U/L

Minimum Detection Limit: 0.003 U/L

Purpose: β-Secretase 1 (BACE 1) Activity Fluorometric Assay Kit

Characteristics: β-Secretase 1 is an aspartic protease, the full name is β-site amyloid precursor protein cleavage enzyme 1(BACE 1). BACE 1 cleaves amyloid precursor protein (APP) to produce amyloid-β peptide (Aβ) of 40 or 42 amino acids. The deposition of Aβ in plaques and vessel walls in the brains of patients with Alzheimer's disease is widely believed to be the major contributor to the disease. The detection principle of this kit is fluorescence resonance energy transfer (FRET) method. A fluorescence donor group and a quenching group are divided at both ends of the substrate. The fluorescence energy is transferred from the fluorescence donor to the quenching group, resulting in the attenuation of the fluorescence intensity of the fluorescence donor molecule itself. When the substrate is cleaved by BACE 1, the quenching group of the substrate and the fluorescent donor are separated and the fluorescent donor is no longer quenched, the fluorescence of the fluorescent donor can be detected, so that the enzymatic activity of BACE 1 can be calculated. The fluorescence produced by this kit had a maximum excitation wavelength of 325 nm and a maximum absorption wavelength of 393 nm.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 4.7-10.4%

Intra-assay precision: 2.2-3.2%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: BACE1 (Beta-secretase 1 (BACE1))

Alternative Name: BACE 1