Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red)

Catalog No. ABIN7849959

Quick Overview for Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red) (ABIN7849959)

Target: ROS1 (C-Ros Oncogene 1 , Receptor tyrosine Kinase (ROS1))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples

Analytical Method: Quantitative

Product Details

Purpose: Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red)

Characteristics: Reactive oxygen species (ROS) refers to the general term of substances that are composed of oxygen in the body or in the natural environment and contain oxygen and are active in nature: there is mainly a kind of excited oxygen molecule, namely singlet oxygen molecule or singlet oxygen molecule. Three oxygen-containing free radicals, namely superoxide anion radicals, hydroxyl radicals, and hydroperoxyradicals, two peroxides, namely hydrogen peroxide and lipid peroxide, and a nitrogen-containing oxide. Dihydroethidium can cross the cell membrane and enter the cell to bind to DNA or RNA, forming hydroethidium to produce red fluorescence. Dihydroethidium is mainly oxidized by superoxide anion type reactive oxygen species in cells. Dihydroethidium itself is blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm. After dehydrogenation, dihydroethidium binds to RNA or DNA to produce red fluorescence with an excitation wavelength of 300 nm or 518 nm and an emission wavelength of 610 nm.

Application Details

Assay Time: 1.5 h

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: ROS1 (C-Ros Oncogene 1 , Receptor tyrosine Kinase (ROS1))

Alternative Name: ROS