Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit

Catalog No. ABIN7849977

Quick Overview for Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit (ABIN7849977)

Target: Cytochrome C Oxidase

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.52 U/L - 30.9 U/L

Minimum Detection Limit: 0.52 U/L

Purpose: Cell Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit

Characteristics: Mitochondrial complex IV, also known as cytochrome C oxidase, is one of the major enzymes in the mitochondrial respiratory chain. It oxidizes the reduced cytochrome C converted from mitochondrial complex III to oxidized cytochrome C and consumes oxygen to generate water. Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.

Application Details

Assay Time: 0.5 h

Assay Precision:

Inter-assay precision: 4.0-9.5%

Intra-assay precision: 3.0-5.5%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: Cytochrome C Oxidase