Ethanol Colorimetric Assay Kit

Catalog No. ABIN7849981

Quick Overview for Ethanol Colorimetric Assay Kit (ABIN7849981)

Target: Ethanol

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Beverages, Plasma, Serum

Analytical Method: Quantitative

Product Details

Detection Range: 0.27 μmol/mL - 10.0 μmol/mL

Minimum Detection Limit: 0.27 μmol/mL

Purpose: Ethanol Colorimetric Assay Kit

Characteristics: Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.

Application Details

Assay Time: 0.5 h

Assay Precision:

Inter-assay precision: 5%

Intra-assay precision: 3.5%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: Ethanol