Fatty Acid Amide Hydrolase (FAAH) Activity Fluorometric Assay Kit

Catalog No. ABIN7849983

Quick Overview for Fatty Acid Amide Hydrolase (FAAH) Activity Fluorometric Assay Kit (ABIN7849983)

Target: FAAH (Fatty Acid Amide Hydrolase (FAAH))

Detection Method: Fluorometric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.004 U/L - 0.105 U/L

Minimum Detection Limit: 0.004 U/L

Purpose: Fatty Acid Amide Hydrolase (FAAH) Activity Fluorometric Assay Kit

Characteristics: Fatty acid amide hydrolase (FAAH) is a membrane binding protein belonging to the serine hydrolase family. FAAH hydrolyzes many important endogenous fatty acid amides, including endocannabinoid (AEA) and palmitoyl glycolamide (PEA). FAAH plays a key role in controlling cannabinoid signaling by hydrolyzing 2arachidenol glycerol in AEA and certain tissues. Detection principle: FAAH specifically cut the substrate, the fluorophores emit fluorescence, and detect fluorescence at the excitation wavelength of 340 nm and the emission wavelength of 440 nm. Because FAAH is a serine protein, FAAHspecific inhibitors are used in the kit to inhibit enzyme activity and exclude the effects of non-specific enzyme activity.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 0.2-12.2%

Intra-assay precision: 3.7-6.0%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: FAAH (Fatty Acid Amide Hydrolase (FAAH))

Alternative Name: Fatty Acid Amide Hydrolase