Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Catalog No. ABIN7850006

Quick Overview for Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (ABIN7850006)

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 1.1 IU/L - 72.3 IU/L

Minimum Detection Limit: 1.1 IU/L

Purpose: Aspartate Aminotransferase (AST/GOT) Activity Assay Kit

Characteristics: AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.

Application Details

Assay Time: 1.5 h

Assay Precision:

Inter-assay precision: 6.8%

Intra-assay precision: 5.3%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: 4 °C

Storage Comment: This product can be stored at 2~8°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Alternative Name: AST