Glutamine (Gln) Colorimetric Assay Kit
Quick Overview for Glutamine (Gln) Colorimetric Assay Kit (ABIN7850009)
Target
Detection Method
Application
Sample Type
Analytical Method
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Detection Range
- 0.036 mmol/L - 2.0 mmol/L
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Minimum Detection Limit
- 0.036 mmol/L
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Purpose
- Glutamine (Gln) Colorimetric Assay Kit
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Characteristics
- Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.
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Assay Time
- 1 h
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Assay Precision
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Inter-assay precision: 4.7%
Intra-assay precision: 3.2%
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- -20 °C
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Storage Comment
- This product can be stored at -20°C for 12 months with shading light.
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Expiry Date
- 12 months
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- Glutamine (GLN)
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Alternative Name
- Gln
Target
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