Glutamine Synthetase (GS) Activity Colorimetric Assay Kit

Catalog No. ABIN7850010

Quick Overview for Glutamine Synthetase (GS) Activity Colorimetric Assay Kit (ABIN7850010)

Target: GLN1 (Glutamine Synthetase (GLN1))

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.25 U/L - 25 U/L

Minimum Detection Limit: 0.25 U/L

Purpose: Glutamine Synthetase (GS) Activity Colorimetric Assay Kit

Characteristics: Glutamine synthetase (GS) is one of the central enzymes in nitrogen metabolism With ATP consumption, GS catalyzes glutamate to produce glutamine. The latter is an amino donor of various nitrogenous substances such as proteins, nucleic acids and various coenzymes. As a conditionally essential amino acid for the human body, the production of glutamine has received extensive attention in recent years. GS catalyzes ammonium ion and glutamic acid to synthesize amide, and produces chromogenic substance under the action of invertase and chromogenic agent. The maximum absorption peak is at 570 nm. The activity of GS in samples is calculated by measuring OD value at 570 nm and standard curve.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 5.3-6.8 %

Intra-assay precision: 2.0-4.0 %

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: GLN1 (Glutamine Synthetase (GLN1))

Alternative Name: Glutamine Synthetase