Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

Catalog No. ABIN7850014

Quick Overview for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit (ABIN7850014)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.49 U/L - 50.00 U/L

Minimum Detection Limit: 0.49 U/L

Purpose: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit

Characteristics: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), a key enzyme in the glycolytic pathway, catalyzes the oxidation of glyceraldehyde-3-phosphate to 1, 3-diphosphoglycerate. This response is closely related to the gluconeogenic pathway, the maintenance of blood glucose concentrations in the body, and the development of diabetes. GAPDH gene is a housekeeping gene, which is expressed at a high level in almost all tissues. Therefore, GAPDH widely exists in tissues and cells and plays an important role in the disorders of glucose, lipid and protein metabolism in the body. This kit can calculate the enzyme activity of GAPDH by measuring the OD value change at 450 nm per unit time of the product produced by GAPDH catalyzing the oxidation of glyceraldehyde-3-phosphate.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 3.2-8.6%

Intra-assay precision: 3.2-5%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH