NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit
Quick Overview for NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit (ABIN7850028)
Target
See all Isocitrate Dehydrogenase (IDH) KitsDetection Method
Application
Sample Type
Analytical Method
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Detection Range
- 0.1 U/L - 50.47 U/L
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Minimum Detection Limit
- 0.1 U/L
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Purpose
- NADP-Isocitrate Dehydrogenase (NADP-IDH) Activity Assay Kit
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Characteristics
- Isocitrate dehydrogenase converts isocitrate to α- ketoglutaric acid, while converting NADP+ to NADPH. Under the action of electron coupling agents, the generated NADPH has a characteristic absorption peak at 450 nm. The activity of NADP-IDH can be calculated by measuring the change of absorbance value at 450 nm.
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Assay Time
- 1 h
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Assay Precision
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Inter-assay precision: 4.3-11.4%
Intra-assay precision: 5.0-6.0%
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- -20 °C
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Storage Comment
- This product can be stored at -20°C for 12 months with shading light.
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Expiry Date
- 12 months
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- Isocitrate Dehydrogenase (IDH)
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Alternative Name
- NADP-Isocitrate Dehydrogenase
Target
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