NAD+/NADH Colorimetric Assay Kit (WST-8)

Catalog No. ABIN7850058

Quick Overview for NAD+/NADH Colorimetric Assay Kit (WST-8) (ABIN7850058)

Target: NAD/NADH

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.02 μmol/L - 5.0 μmol/L

Minimum Detection Limit: 0.02 μmol/L

Purpose: NAD+/NADH Colorimetric Assay Kit (WST-8)

Characteristics: Detect total content of NAD+ and NADH Ethanol generates acetaldehyde under the action of enzyme. Meanwhile, NAD+ is reduced to NADH, NADH, under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, the total content of NAD+ and NADH can be quantified by measure the OD value at 450 nm. Detect NADH After treating sample, heat at 60oC water bath for 30 min. the NAD+ of the sample is decomposed and only NADH remains. NADH reduces WST-8 to form yellow product, and the amount of NADH is determined by measure the OD value at 450 nm. Detect NAD+ and NAD+/NADH The content of NAD+ and the ratio of NAD+/NADH in the sample can be obtained according to the total content of NAD+ and NADH obtained of the first two steps as well as the separate content of NADPH. Note: NADP+ and NADPH have no effect on the determination results.

Application Details

Assay Time: 1 h

Assay Precision:

Inter-assay precision: 9.1%

Intra-assay precision: 1.8%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: NAD/NADH

Alternative Name: NAD+/NADH

Target Type: Chemical